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   2024-04-10 14:36:12 by Jason Bacon | Files touched by this commit (5)
Log message:
biology/minimap2: Update to 2.28

Fix --MD flag
Add experimental lr:hqae

Changes: https://github.com/lh3/minimap2/releases
Reported by: portscout
   2024-03-13 14:54:04 by Jason Bacon | Files touched by this commit (6)
Log message:
biology/minimap2: Update to 2.27

Several new features and bug fixes since 2.24

Changes: https://github.com/lh3/minimap2/releases
Reported by: portscout
   2023-08-14 07:25:36 by Thomas Klausner | Files touched by this commit (1247)
Log message:
*: recursive bump for Python 3.11 as new default
   2023-07-09 10:37:39 by Nia Alarie | Files touched by this commit (2)
Log message:
minimap2: Honour CFLAGS/LDFLAGS. Hopefully fixes build on platforms
where zlib is installed from pkgsrc.
   2022-03-13 09:05:07 by Makoto Fujiwara | Files touched by this commit (2)
Log message:
(biology/minimap2) Updated 2.18 to 2.24

Release 2.24-r1122 (26 December 2021)
-------------------------------------

This release improves alignment around long poorly aligned regions. Older
minimap2 may chain through such regions in rare cases which may result in
missing alignments later. The issue has become worse since the the change of
the chaining algorithm in v2.19. v2.23 implements an incomplete remedy. This
release provides a better solution with a X-drop-like heuristic and by enabling
two-bandwidth chaining in the assembly mode.

(2.24: 26 December 2021, r1122)

Release 2.23-r1111 (18 November 2021)
-------------------------------------

Notable changes:

 * Bugfix: fixed missing alignments around long inversions (#806 and #816).
   This bug affected v2.19 through v2.22.

 * Improvement: avoid extremely long mapping time for pathologic reads with
   highly repeated k-mers not in the reference (#771). Use --q-occ-frac=0
   to disable the new heuristic.

 * Change: use --cap-kalloc=1g by default.

(2.23: 18 November 2021, r1111)

Release 2.22-r1101 (7 August 2021)
----------------------------------

When choosing the best alignment, this release uses logarithm gap penalty and
query-specific mismatch penalty. It improves the sensitivity to long INDELs in
repetitive regions.

Other notable changes:

 * Bugfix: fixed an indirect memory leak that may waste a large amount of
   memory given highly repetitive reference such as a 16S RNA database (#749).
   All versions of minimap2 have this issue.

 * New feature: added --cap-kalloc to reduce the peak memory. This option is
   not enabled by default but may become the default in future releases.

Known issue:

 * Minimap2 may take a long time to map a read (#771). So far it is not clear
   if this happens to v2.18 and earlier versions.

(2.22: 7 August 2021, r1101)

Release 2.21-r1071 (6 July 2021)
--------------------------------

This release fixed a regression in short-read mapping introduced in v2.19
(#776). It also fixed invalid comparisons of uninitialized variables, though
these are harmless (#752). Long-read alignment should be identical to v2.20.

(2.21: 6 July 2021, r1071)

Release 2.20-r1061 (27 May 2021)
--------------------------------

This release fixed a bug in the Python module and improves the command-line
compatibiliity with v2.18. In v2.19, if `-r` is specified with an `asm*` preset,
users would get alignments more fragmented than v2.18. This could be an issue
for existing pipelines specifying `-r`. This release resolves this issue.

(2.20: 27 May 2021, r1061)

Release 2.19-r1057 (26 May 2021)
--------------------------------

This release includes a few important improvements backported from unimap:

 * Improvement: more contiguous alignment through long INDELs. This is enabled
   by the minigraph chaining algorithm. All `asm*` presets now use the new
   algorithm. They can find INDELs up to 100kb and may be faster for
   chromosome-long contigs. The default mode and `map*` presets use this
   algorithm to replace the long-join heuristic.

 * Improvement: better alignment in highly repetitive regions by rescuing
   high-occurrence seeds. If the distance between two adjacent seeds is too
   large, attempt to choose a fraction of high-occurrence seeds in-between.
   Minimap2 now produces fewer clippings and alignment break points in long
   satellite regions.

 * Improvement: allow to specify an interval of k-mer occurrences with `-U`.
   For repeat-rich genomes, the automatic k-mer occurrence threshold determined
   by `-f` may be too large and makes alignment impractically slow. The new
   option protects against such cases. Enabled for `asm*` and `map-hifi`.

 * New feature: added the `map-hifi` preset for maping PacBio High-Fidelity
   (HiFi) reads.

 * Change to the default: apply `--cap-sw-mem=100m` for genomic alignment.

 * Bugfix: minimap2 could not generate an index file with `-xsr` (#734).

This release represents the most signficant algorithmic change since v2.1 in
2017. With features backported from unimap, minimap2 now has similar power to
unimap for contig alignment. Unimap will remain an experimental project and is
no longer recommended over minimap2. Sorry for reverting the recommendation in
short time.

(2.19: 26 May 2021, r1057)
   2021-10-26 12:03:45 by Nia Alarie | Files touched by this commit (73)
Log message:
biology: Replace RMD160 checksums with BLAKE2s checksums

All checksums have been double-checked against existing RMD160 and
SHA512 hashes
   2021-10-07 15:19:44 by Nia Alarie | Files touched by this commit (73)
Log message:
biology: Remove SHA1 hashes for distfiles
   2021-05-29 19:35:18 by Brook Milligan | Files touched by this commit (2)
Log message:
biology/minimap2: install minimap2 program instead of python binding

The distfile for minimap2 includes two different components: (i) the
minimap2 sequence mapping program itself, and (ii) a python binding
generally referred to as mappy.  The initial version of this package
included only the python binding.  However, it is more appropriate
that the minimap2 package should contain the program of the same name,
and a new package be created with the name mappy for the python
binding.  Splitting these into two packages makes sense, because this
allows users to install the minimap2 package without python
dependencies.
   2021-05-26 20:49:20 by Brook Milligan | Files touched by this commit (4)
Log message:
biology/minimap2: add minimap 2.18

## Users' Guide

Minimap2 is a versatile sequence alignment program that aligns DNA or
mRNA sequences against a large reference database. Typical use cases
include: (1) mapping PacBio or Oxford Nanopore genomic reads to the
human genome; (2) finding overlaps between long reads with error rate
up to ~15%; (3) splice-aware alignment of PacBio Iso-Seq or Nanopore
cDNA or Direct RNA reads against a reference genome; (4) aligning
Illumina single- or paired-end reads; (5) assembly-to-assembly
alignment; (6) full-genome alignment between two closely related
species with divergence below ~15%.

For ~10kb noisy reads sequences, minimap2 is tens of times faster than
mainstream long-read mappers such as BLASR, BWA-MEM, NGMLR and
GMAP. It is more accurate on simulated long reads and produces
biologically meaningful alignment ready for downstream analyses. For
>100bp Illumina short reads, minimap2 is three times as fast as
BWA-MEM and Bowtie2, and as accurate on simulated data.  Detailed
evaluations are available from the minimap2 paper or the preprint.

Release 2.18-r1015 (9 April 2021)
---------------------------------

This release fixes multiple rare bugs in minimap2 and adds additional
functionality to paftools.js.

Changes to minimap2:

 * Bugfix: a rare segfault caused by an off-by-one error (#489)

 * Bugfix: minimap2 segfaulted due to an uninitilized variable (#622 and #625).

 * Bugfix: minimap2 parsed spaces as field separators in BED (#721). This led
   to issues when the BED name column contains spaces.

 * Bugfix: minimap2 `--split-prefix` did not work with long reference names
   (#394).

 * Bugfix: option `--junc-bonus` didn't work (#513)

 * Bugfix: minimap2 didn't return 1 on I/O errors (#532)

 * Bugfix: the `de:f` tag (sequence divergence) could be negative if there were
   ambiguous bases

 * Bugfix: fixed two undefined behaviors caused by calling memcpy() on
   zero-length blocks (#443)

 * Bugfix: there were duplicated SAM @SQ lines if option `--split-prefix` is in
   use (#400 and #527)

 * Bugfix: option -K had to be smaller than 2 billion (#491). This was caused
   by a 32-bit integer overflow.

 * Improvement: optionally compile against SIMDe (#597). Minimap2 should work
   with IBM POWER CPUs, though this has not been tested. To compile with SIMDe,
   please use `make -f Makefile.simde`.

 * Improvement: more informative error message for I/O errors (#454) and for
   FASTQ parsing errors (#510)

 * Improvement: abort given malformatted RG line (#541)

 * Improvement: better formula to estimate the `dv:f` tag (approximate sequence
   divergence). See DOI:10.1101/2021.01.15.426881.

 * New feature: added the `--mask-len` option to fine control the removal of
   redundant hits (#659). The default behavior is unchanged.

Changes to mappy:

 * Bugfix: mappy caused segmentation fault if the reference index is not
   present (#413).

 * Bugfix: fixed a memory leak via 238b6bb3

 * Change: always require Cython to compile the mappy module (#723). Older
   mappy packages at PyPI bundled the C source code generated by Cython such
   that end users did not need to install Cython to compile mappy. However, as
   Python 3.9 is breaking backward compatibility, older mappy does not work
   with Python 3.9 anymore. We have to add this Cython dependency as a
   workaround.

Changes to paftools.js:

 * Bugfix: the "part10-" line from asmgene was wrong (#581)

 * Improvement: compatibility with GTF files from GenBank (#422)

 * New feature: asmgene also checks missing multi-copy genes

 * New feature: added the misjoin command to evaluate large-scale misjoins and
   megabase-long inversions.

Although given the many bug fixes and minor improvements, the core algorithm
stays the same. This version of minimap2 produces nearly identical alignments
to v2.17 except very rare corner cases.

Now unimap is recommended over minimap2 for aligning long contigs against a
reference genome. It often takes less wall-clock time and is much more
sensitive to long insertions and deletions.

(2.18: 9 April 2021, r1015)

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